• 专利附录
  • 专利说明
  • 权利要求
  • 类似技术
  • 同族专利
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  • 法律状态
  • 优先权
    • 专利摘要:
    Apost-harvest processing method of G. rigescens includes the following steps: cleaning fresh harvested G. rigescens with water to remove soil, removing the aboveground part of G. rigescens, blanching the root of G. rigescens in hot water at 40-70°C for 1-5 min, and then putting the blanched root of G. rigescens into an oven to dry until a moisture content reaches the pharmacopoeia standard, i.e., 39.0%, to obtain a G. rigescens medicinal material. Compared with the traditional method, the new method can increase the gentiopicroside content by 9.30% and sweroside content by 14.29% in G. rigescens. Compared with the current heating, microwave and other treatments, the new operation steps are simpler, the production cost is lower, which makes the new method especially suitable for farmers to perform independently.
    公开号:NL2027660A
    申请号:NL2027660
    申请日:2021-02-26
    公开日:2021-10-20
    发明作者:He Daiqin；Wu Xinyi；Yi Xia；Qu Yunhui；Tian Hao；Li Hong
    申请人:Agro Products Processing Res Institute Yaas；
    IPC主号:
    专利说明:

    -1- Post-harvest Processing Method of Gentiana rigescens Technical Field The present invention belongs to the technical field of agricultural product processing, and in particular, to a post-harvest processing method of Gentiana rigescens. Background Gentiana rigescens Franch. (G. rigescens) 1s a perennial herb of the family Gentianaceae. G. rigescens is used as medicine with roots and rhizomes, and is one of the original plants of the Chinese medicine gentian. G. rigescens has several medicinal effects, such as anti-inflammatory and analgesic, anti-apoptosis, prevention of liver failure, cholagogic, antibacterial, and others. The main active components are loganin acid, sweroside, swertiamarin, gentiopicroside and other secoiridoid compounds. Iridoids have poor stability and are susceptible to degradation due to environmental factors. Folk post-harvest drying often involves removing stems and leaves after harvesting, washing the soil, and drying them in the sun or in the shade. The operation is random and the environmental factors inconsistent, which causes the active ingredient content to change significantly during post-harvest drying.
    By comparing the contents of gentiopicroside in original medicinal materials, shade-dried medicinal materials, natural sun-dried medicinal materials and kiln-dried medicinal materials stored for one month, XU Qiuxia et al. found that different treatment methods could lead to the degradation of gentiopicroside, among which the loss of gentiopicroside in shade-dried G. rigescens was the smallest, the loss of gentiopicroside in natural sun-dried G. rigescens was the largest, and the content of gentiopicroside in kiln-dried samples was between shade-dried and natural sun-dried samples (XU Qiuxia et al, China Pharmacy, 2013).
    WANG Xijun et al. used three methods of kiln drying (45°C, 55°C, 65°C), outdoor drying and indoor drying in the shade to treat Gentiana scabra. Through the detection of gentiopicroside in G. scabra, it was found that the content of gentiopicroside in indoor-dried G. scabra was the highest, followed by the content of gentiopicroside in sun-dried samples, and the least in the kiln-dried samples (WANG Xijun et al., Journal of China Pharmaceutical, 2004). Therefore, it can be seen that remarkable degradation
    -2- of the effective components in G. rigescens during the post-harvest drying process directly affects the quality of G. rigescens medicinal materials, resulting in poor efficacy.
    Currently, ZUO Zhitian et al. compared the content of active ingredients in G. rigescens dried by nine treatment methods, including drying in the shade and drying in the shade after blanching. The results showed that the content of gentiopicroside in shade-dried GC. rigescens was the highest (ZUO Zhitian, Jowrnal of Southwest Agriculture, 2017). Through previous studies, it is known that drying in the shade is beneficial to the accumulation of gentiopicroside, but it takes a long time. According to field surveys, drying in the shade takes 14-30 days. Therefore, the work efficiency is low, and it is difficult to implement on a large scale due to site restrictions, and large areas of mildew will occur in rainy weather.
    WANG Cong’s research showed that microwave vacuum drying can reduce the loss of effective components in G. rigescens (WANG Cong, Shaanxi Agricultural Sciences, 2016). However, microwave drying requires equipment investment. Production costs are too high for farmers in producing areas to implement on a large scale.
    RAO Gaoxiong et al. showed that the loss of gentiopicroside could be reduced by exposure to the sun or baking at 80°C (RAO Gaoxiong et al., Journal of Yunnan University of Traditional Chinese Medicine, 2002). However, during the harvest season of G. rigescens in Yunnan, there are many rainy weather, and it is difficult to achieve the conditions for all the medicinal materials to be exposed to the sun during the harvest period. Although heating and drying can be achieved by building a barn, the baking temperature in the self-built barn is generally 50-70°C according to the survey, which is difficult to reach the high temperature condition of 80°C. Therefore, it is of far-reaching significance to explore a “refined” post-harvest drying processing method that is easy for farmers to perform independently for the preparation of high-quality Chinese medicine raw materials and Chinese medicine preparations with stable therapeutic effect.
    Summary The present invention aims to overcome the deficiencies of the prior art and provide a post-harvest processing method of G. rigescens.
    In order to achieve the above objective, the technical solution provided by the
    -3- present invention is as follows.
    A post-harvest processing method of G. rigescens, including the following steps: cleaning fresh harvested (5. rigescens with water to remove soil, removing the aboveground part of G. rigescens, blanching the root of G. rigescens in hot water at 40- 70°C, preferably 40°C for 1-5 min, preferably 3.5-5.0 min, and then putting the blanched root of G. rigescens into an oven to dry until a moisture content is <9.0% (the moisture content reaches the pharmacopoeia standard), to obtain a G. rigescens medicinal material.
    Preferably, a temperature in the oven is 40-70°C.
    More preferably, the temperature in the oven is 60°C.
    Compared with the prior art, the present invention has the following advantages.
    1. Aiming at the serious loss of main active ingredient gentiopicroside during the primary processing of G. rigescens, the invention combines blanching and drying treatment organically in the post-harvest processing process of G. rigescens through experimental exploration, and has achieved obvious effects.
    2. Previous studies showed that the content of gentiopicroside in G. rigescens with different treatments was indoor drying in the shade > outdoor drying > kiln drying. The combination of blanching and drying treatment can not only effectively improve the retention rate of gentiopicroside, but also can increase the content of gentiopicroside and sweroside by 9.30% and 14.29%, respectively, compared with that in shade-dried G. rigescens.
    3. Previous studies showed that drying at 80°C can reduce the degradation of gentiopicroside. Through the operation of blanching, the drying temperature can be reduced and the production cost can be reduced.
    4. Compared with the heating and microwave treatment, the operation steps of the present invention are simpler and the production cost is lower, which are suitable for farmers.
    In conclusion, compared with the traditional method, the new method can increase the gentiopicroside content by 9.30% and sweroside content by 14.29% in G. rigescens.
    Compared with the current heating, microwave and other treatment, the new operation steps are simpler, the production cost is lower, which are suitable for farmers to operate.
    -4- Detailed Description of the Embodiments Example 1 A total of 5 groups of fresh harvested G. rigescens are collected, each with 5 samples, cleaned with water to remove soil, and the aboveground part is removed. The root of G. rigescens is blanched in hot water at 40°C for 5 min. After being taken out, the root is dried in an oven at 60°C until dry to obtain G. rigescens medicinal materials. The contents of active ingredients in G. rigescens detected by high performance liquid chromatography (HPLC) are as follows: the content of loganin acid is 0.505 + 0.008%, the content of swertiamarin is 0.243 + 0.004%, the content of gentiopicroside is 7.290 +
    0.065% and the content of sweroside is 0.112 + 0.019%. The contents of loganin acid, swertiamarin, gentiopicroside and sweroside in traditional shade-dried G. rigescens are
    0.502 + 0.003%, 0.242 + 0.005%, 6.667 + 0.015% and 0.098 + 0.006%, respectively. The content of gentiopicroside and sweroside of the present example increase by 9.30% and 14.29%, respectively. Example 2 A total of 5 groups of fresh harvested G. rigescens are collected, each with 5 samples, cleaned with water to remove soil, and the aboveground part is removed. The root of (5. rigescens is blanched in hot water at 50°C for 1 min. After being taken out, the root is dried in an oven at 70°C until dry to obtain G. rigescens medicinal materials. The contents of active ingredients in G. rigescens detected by HPLC are as follows: the content of loganin acid is 0.502 + 0.078%, the content of swertiamarin is 0.265 +
    0.002%, the content of gentiopicroside is 6.807 + 0.075% and the content of sweroside is 0.100 + 0.009%. The contents of loganin acid, swertiamarin, gentiopicroside and sweroside in traditional shade-dried G. rigescens are 0.500 + 0.002%, 0.241 + 0.005%,
    6.659 + 0.008% and 0.095 + 0.005%, respectively. The content of gentiopicroside, sweroside and swertiamarin of the present example increase by 2.22%, 5.26% and
    9.90%, respectively. Example 3 A total of 5 groups of fresh harvested G. rigescens are collected, each with 5 samples, cleaned with water to remove soil, and the aboveground part is removed. The root of (5. rigescens is blanched in hot water at 70°C for 3.88 min. After being taken out, the root is dried in an oven at 40°C until dry to obtain G. rigescens medicinal materials.
    -5- The contents of active ingredients in G. rigescens detected by HPLC are as follows: the content of loganin acid is 0.519 + 0.010%, the content of swertiamarin is 0.240 +
    0.006%, the content of gentiopicroside is 6.800 + 0.090% and the content of sweroside is 0.099 + 0.003%. The contents of loganin acid, swertiamarin, gentiopicroside and sweroside in traditional shade-dried (5. rigescens are 0.497 + 0.008%, 0.239 + 0.004%,
    6.676 + 0.004% and 0.090 + 0.001%, respectively. The content of loganin acid, gentiopicroside and sweroside of the present example increase by 4.43%, 1.86% and
    10.00%, respectively. Example 4 (1) Blanching + kiln drying group A total of 5 groups of fresh harvested G. rigescens are collected, each with 5 samples, cleaned with water to remove soil, and the aboveground part is removed. The root of G. rigescens is blanched in hot water at 40°C for 5 min. After being taken out, the root is dried in an oven at 60°C until dry to obtain G. rigescens medicinal materials. (2) Blanching + shade drying group A total of 5 groups of fresh harvested G. rigescens are collected, each with 5 samples, cleaned with water to remove soil, and the aboveground part is removed. The root of G. rigescens is blanched in hot water at 40°C for 5 min. After being taken out, the root is put into a dark place indoors to dry in the shade to obtain G. rigescens medicinal materials. (3) Kiln drying group A total of 5 groups of fresh harvested G. rigescens are collected, each with 5 samples, cleaned with water to remove soil, and the aboveground part is removed. The root of G. rigescens 1s dried in an oven at 60°C until dry to obtain G. rigescens medicinal materials. (4) Blanching + sun drying group A total of 5 groups of fresh harvested G. rigescens are collected, each with 5 samples, cleaned with water to remove soil, and the aboveground part is removed. The root of G. rigescens is blanched in hot water at 40°C for 5 min. After being taken out, the root is exposed to the sun outside to obtain G. rigescens medicinal materials. (5) Sun drying group A total of 5 groups of fresh harvested G. rigescens are collected, each with 5 samples, cleaned with water to remove soil, and the aboveground part is removed. The
    -6- root of (5. rigescens is exposed to the sun outside to obtain G. rigescens medicinal materials. (6) Shade drying group A total of 5 groups of fresh harvested G. rigescens are collected, each with 5 samples, cleaned with water to remove soil, and the aboveground part is removed.
    The root of (7. rigescens is placed in a dark place indoors to dry in the shade to obtain G. rigescens medicinal materials.
    The results are shown in Table 1. Table 1 Time/da Loganin Swertiamari | Gentiopicrosid | Sweroside Treatment acid 07 0 0 y 0 n content/% e content/% content/% content/% Blanchin . St + g + kiln 1/3 930 0.00 0.243+0.004 7.290+0.065 oH 0.01 drying Blanchin + + g + shade 15 020 0.00 0.098+0.004 4.087+0.032 002 0.00 drying i + + Kiln 724 | 94933901 020010,002 | 5.99540.041 | 9:009=0.00 drying 7 8 Blanchin 30+ + g + sun 3 990 0.01 6.210+0.001 6.090+0.004 0.060: 0.04 drying u 2+ + Sun 3 0.462: 0.00 0.224+0.004 6.310+0.080 0.079+0.01 drying 6 1 502+ oo 08+ Shade 15 0502000 242+0.005 | 6.667+0.015 | 9:098=0-00 drying 3 6
    权利要求:
    Claims (5)
    [1]
    A method for post-harvest processing of Gentiana rigescens (G. rigescens), the method comprising the steps of: cleaning freshly harvested CG with water. rigescens to remove soil, remove an above-ground part of G. rigescens, perform blanching treatment on a root of G. rigescens in warm water at 40 - 70°C for 1 - 5 min, and then put it in an oven placing a blanched root of G. rigescens to dry to a moisture content < 9.0% to obtain a medicinal material of G. rigescens.
    [2]
    A method according to claim 1, wherein a temperature of the hot water is 40°C 1s.
    [3]
    The method of claim 1, wherein a time of the blanching treatment is 3.5-5.0 min.
    [4]
    The method of claim 1, wherein a temperature in the oven is 40-70°C.
    [5]
    A method according to claim 4, wherein the temperature in the oven is 60°C.
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    同族专利:
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    引用文献:
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    法律状态:
    优先权:
    申请号 | 申请日 | 专利标题
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